streptavidin biotin complex sabc immunohistochemistry staining kit  (Boster Bio)

Journal: Frontiers in Immunology

Article Title: B and T lymphocyte attenuator (BTLA) and PD-1 pathway dual blockade promotes antitumor immune responses by reversing CD8 + T-cell exhaustion in non-small cell lung cancer

doi: 10.3389/fimmu.2025.1553042

Figure Lengend Snippet: Abnormally increased infiltration of BTLA + CD8 + T cells in NSCLC tumor microenvironment (A) Expression of BTLA on lymphocytes in different kinds of specimens from NSCLC patient was evaluated by flow cytometry. (B) Representative flow plots and statistics of BTLA expression on CD8 + T cells from NSCLC and benign pleural effusions. (C) Representative flow plots and statistics of BTLA expression on CD8 + T cells from NSCLC and nontumor tissues. (D) Representative flow plots and statistics of BTLA expression on CD8 + T cells from NSCLC patients and health donor peripheral blood. Data are presented as mean ± SEM. P values were calculated by two-tailed unpaired Student’s t -test. (E, F) Expression of HVEM in tumor and paired tumor-adjacent tissues of NSCLC patients was analyzed by IHC staining, HVEM positive cells were quantified by IHC score, scale bar, 100 μm. n=9. P values were calculated by two-tailed paired Student’s t-test. (*, P < 0.05; **, P < 0.01; ***, P < 0.001).

Article Snippet: The appropriate secondary antibodies were applied, followed by incubation with streptavidin–biotin complex (SABC) using the SABC immunohistochemical kit (BOSTER).

Techniques: Expressing, Flow Cytometry, Two Tailed Test, Immunohistochemistry